SOP on Personnel Entry to Microbiology Room 

1.0 Purpose: This SOP describe a procedure for precautions to be taken at the time of entering in microbiology room.

2.0 Scope: Hygienic behaviour maintains the aseptic area clean and keep the microbial load to minimum in the room. 

3.0 Responsibility: Quality Control Microbiologist to ensure that the procedure is followed.

4.0 Materials and Equipment: 

Sterile garments – Coat, mask, disposable cap, gloves

5.0 Procedure:

5.1 Staff should not move about unnecessarily in microbiology room because movement increases bacterial shedding.

5.2 Aseptic area garments should be stored and handled after cleaning and sterilizing in such a manner as to minimise microbial contamination.

5.3 Nails of persons working in the microbiology room should be regularly trimmed.

5.4 Street shoes to be changed each time a person enters the aseptic room.

5.5 The persons should wash hands thoroughly with a disinfectant scrub before wearing sterile gloves.

5.6 The persons should wear a sterile coat, mask and disposable cap.

5.7 The number of persons working in aseptic area should be kept to minimum practicable.