SOP on Calibration of Gas Chromatograph

 Calibration of Gas Chromatograph(GC)

1.0    PURPOSE  

         The purpose of the standard operating procedure is to define a method to calibrate the

         Gas Chromatography (GC).

2.0    SCOPE:   

         This standard operating procedure is applicable to the staff responsible for operation

         and calibration of the Gas Chromatography (GC).


3.1    Section Incharge

3.2    Technical Manager

3.3    Q.A. Manager 

4.0      PROCEDURE:


4.1.1   Instrument to be used

4.1.2    Hydrogen Cylinder

4.1.3    Air Cylinder

4.1.4    Calibrated Temperature Probe

4.1.5    Ethanol

4.1.6    Glassware


Using the standard operating conditions start the carrier gas flow through at a set


Allow the gas to pass through the gas flow meter supplied with the instrument and record the gas flow rate.

Similarly allow the hydrogen gas and air gas to pass through at a set point flow rate of 30ml/minute and 300ml/minute respectively. The gases are allowed to pass through the gas flow meter and the flow rates recorded.

Correlate the observed gas flow rates with the set point flow rates and make adjustments, if necessary. 


Insert a calibrated temperature probe into the oven near the oven sensor. Allow all zones to stabilize and the to become ready. Record the actual temperature probe value for oven temperature.

4.4    Detector/Auto sampler Reproducibility

4.4.1 Repeatability (Precision)

Repeatability will look at the standard deviation of 6 injections of the same concentration of the 0.5% solution of ethanol. From the standard deviation the RSD is calculated. If the standard deviation is less than or equal to 2.0 % the test passes. The purpose of Repeatability is to verify that the Autosampler can repeatedly inject the same volume of sample numerous times. Inject 6 injections of 1mL each.


Linearity will check that the Autosampler can accurately inject different concentration of ethanol. We do this by injecting multiple samples of different concentrations. The injection volume is 1mL from 0.40%, 0.45%, 0.50%, 0.55% and 0.6 % w/v solution of ethanol. There should be linear be a linear relationship between sample concentration and area under the peak.

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