SOP on Calibration of Inductively Coupled Plasma Mass Spectrometer

 Calibration of Inductively Coupled Plasma Mass Spectrometer

1.0 PURPOSE   

The purpose of the standard operating procedure is to provide guidelines for calibration of

      Inductively Coupled Plasma Mass Spectrometer.

2.0 SCOPE  

      The scope of this standard operating procedure includes calibration of Inductively Coupled

       Plasma Mass Spectrometer.


3.1   Application Scientist

3.2   Technical-Manager

3.3    Q.A. Manager


4.1 Argon Gas Cylinders

4.2   Elan 9000 Hardware Guide

4.3   Elan Inductively Coupled Plasma Mass Spectroscopy safety manual

4.4   Tuning Solution


5.1 Precaution while start-up the Inductivel Coupled Plasma Mass Spectrometer

5.1.1  Argon gas cylinder should not be used below 100 psi in term of gas quantity

5.1.2   Release gas pressure should be between 60-80 psi.

5.2  Switch on the blower & recirculater.

5.3  Before the ignition of the plasma, vacuum pressure should be 10-6 torr.

5.4  Open Software.           

5.4.1  Open “Instrument “---------Start Plasma

5.4.1  Leave the plasma for 30 minutes or more (minimum 25 minutes).

5.4.2      Open ----“ Workspace” ----------“Daily Performance .wrk”

5.4.3      Place the tuning solution.

5.4.4      Analyse the above solution as “Sample “.

5.4.5      Do  Daily Performance Check  Mg intensity > 40,000 cps/10 ppb  In Intensity > 250 , 000 cps/10 ppb  U intensity > 200,000 cps/10 ppb  CeO/Ce ;Ba++/Ba < 3 %  Background 220 <2 cps

5.4.6      If above test is not OK then optimize. Optimisation is required only when the value are highly disturbed .Do optimization while referring to the manual. following parameters must be optimized.  Nebuliser Gas Flow (NEB)  Auxiliary Gas Flow  Plasma Gas Flow  Lens Voltage  Inductively Coupled Plasma Radio-frequency (Rf) power  Analog Stage voltage  Pulse Stage voltage  Quadropole Rod Offset (QRD)  Cell Path Voltage (CPV) 

5.4.7      Click on get Analyte list Icon & select Analyte from Analyte Icon i.e.Mg 

5.4.8      Select optimization criteria & select maximum intensity.

5.4.9      Click on optimize icon. 

5.4.10   Repeat the step 5.4.8 , 5.4.9 & 5.4.10  for above mentioned parameters individually i.e. Nebuliser

             Gas Flow, Auxiliary Gas Flow, and Plasma Gas flow


6.1   Working Instructions (Inductively Coupled Plasma Mass Spectrometer); Issue date: Jan  2006

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