SOP on Calibration of Refractometer

 Calibration of Refractometer



The purpose of the standard operating procedure is to provide guidelines for calibration of Refractometer.

2.0  SCOPE 

The scope of this standard operating procedure includes calibration of Refractometer.


3.1    Section Incharge

3.2    Technical Manager

3.3    Q.A. Manager


4.1  The Refractometer is calibrated with different liquids or glass test piece of known refractive index.

4.2  Ensure that the surface of the prisms is cleaned each time either with water or acetone

       before using for the determination of the refractive index.

4.3  Put in a few drops of the reference liquid of known refractive index in between the two prisms.

4.4  Circulate the water from a separate thermostatic bath within the prism box using the inlet

       and outlet tubes provided.

4.5  A thermometer to measure the temperature is provided along with the instrument. By

       continuously circulating the water a fixed desired temperature can be obtained.

4.6  Make the achromatic critical line coincide with the index line with the field of view of the  telescope.

4.7  Keeping the telescope in the position take the reading of the scale against the index mark.

       Record the observed refractive index reading from the scale and correlate the same with

       the reference reading.

4.8  Adjustment can be made by setting the scale reading to correct value of refractive index to

       that fixed temperature by operating the pin provided over the cone of the telescope till the

       critical line moves and coincides with the cross line.

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