Pharmaceutical Microbiology Laboratory Manual - B. Pharma Third Semester PDF

Pharmaceutical Microbiology Laboratory Manual 

LIST OF EXPERIMENTS

1.  Study of equipment and apparatus used in experimental microbiology

2.  Sterilization of glass ware

3.  Preparation   of   media   (nutrient   stabs   and   slants   preparations)   and sterilization

4.  Sub culturing of bacteria and fungus

5.  Staining techniques – Simple staining; Gram’s staining; Negative staining; Acid-fast staining

6.  Study of motility characters

7.  Study of the methods of isolation of pure culture

8.  Bio chemical testing for the identification of micro‐organisms.

9.  Sterility testing for powders and liquids

10.Microbiological assay of antibiotics by cup plate method 

REGULAR PRACTICAL ASSESSMENT SCHEME

 

Sl. no.	Parameters	Marks allotted
01	Maintenance of practical manual book	01
02	Record book neatness and timely submission	02
03	Practical Skills	02
04	Result analysis	01
05	Viva voce	02
06	Attendance and discipline	02
Total		10

Laboratory Safety: Do’s and Don’ts

1.   Wash your hands with disinfectant soap when you arrive at the lab and again before you leave.

2.   Absolutely  no  food,  drinks,  chewing  gum  is  allowed  in  the  laboratory.  Do  not  put anything in your mouth such as pencils, pens, labels, or fingers. Do not store food in areas where microorganisms are stored.

3.   Avoid loose fitting items of clothing. Wear appropriate shoes/sandals in the laboratory.

4.   Keep your hands away from your mouth and eyes.

5.   Keep your workspace free of all unnecessary materials. Backpacks, purses should be placed in the appropriate place.

DO NOT KEEP YOUR BAGS ON WORKING TABLE.

6.   Disinfect work areas before and after use with 70% ethanol or fresh 10% bleach.

7.   Laboratory   equipment   and   work   surfaces   should   be   decontaminated   with   an appropriate disinfectant on a routine basis, and especially after spills, splashes, or other contamination.

8.   Label everything clearly.

9.   Replace caps on reagents, solution bottles, and bacterial cultures. Do not open Petri dishes in the lab unless absolutely necessary.

10. Inoculating loops and needles should be flame sterilized in a Bunsen burner before you lay them down. Do not walk around in the laboratory with a loop or pipette having infectious materials.

11. Turn off Bunsen burners when not in use.

12. When you flame sterilize  with alcohol, be  sure that you do not have  any papers under You.

13. Treat all microorganisms as potential pathogens. Use appropriate care and do not take cultures out of the laboratory. 

14. Wear disposable gloves when working with  potentially infectious microbes or samples. If  you  are  working  with  a  sample  that  may  contain  a  pathogen,  then  be  extremely careful to use good bacteriological technique. 

15. Sterilize equipment and materials. 

16. Never pipette by mouth. Use a pipetting aid or adjustable volume pipettes.  

17. Consider  everything  a  biohazard.  Do  not  pour  anything  down  the  sink.  Autoclave liquids and broth cultures to sterilize them before discarding. 

18. Dispose of all solid waste material in a biohazard bag and autoclave it before discarding. 

19. Report spills and accidents immediately to your instructor. Clean small spills with care and seek help for large spills. 

20. Report all injuries or accidents immediately to the instructor, no matter how small they seem.

21. Students  are  not  allowed  to  touch  any  equipment,  chemicals  or  other materials in the laboratory area until you are instructed by Teacher or Technician.  

22. Before starting Laboratory work follow all written and verbal instructions carefully.  

23. If you do not understand a direction or part of a procedure, ASK YOUR CONCERN TEACHER BEFORE PROCEEDING WITH THE ACTIVITY.  

24. Before use equipment must be read carefully Labels and instructions. Set up and use the equipment as directed by your teacher.

25. If you do not understand how to use a piece of equipment, ASK THE TEACHER FOR HELP!

REQUIREMENTS FOR PRACTICALS

1.   Practical Manual

2.   Clean Apron

3.   White Mask and Cap

4.   Completed neat record

5.   Inoculation loop

6.   Match box / Lighter

7.   Ball pens- Red, Blue, Black and Green pens

8.   Pencil, Eraser, Scale, Sharpener

9.   Colour pencils

10. Sticker labels

11. Scissors

12. Glue

13. Butter paper

14. Spatula

15. Wiping cloth

16. Calculator

17. Medical Dictionary

18. Syllabus copy

Pharmaceutical Microbiology Laboratory Manual