BP 605 T. PHARMACEUTICAL BIOTECHNOLOGY (Theory)
Scope:
Biotechnology has a long promise to revolutionize the
biological sciences and technology.
Scientific application of biotechnology in the field of
genetic engineering, medicine and fermentation technology makes the subject
interesting.
Biotechnology is leading to new biological revolutions in
diagnosis, prevention and cure of diseases, new and cheaper pharmaceutical
drugs.
Biotechnology has already produced transgenic crops and
animals and the future promises lot more. It is basically a research-based
subject.
Objectives: Upon
completion of the subject student shall be able to;
1. Understanding the
importance of Immobilized enzymes in Pharmaceutical Industries
2. Genetic
engineering applications in relation to production of pharmaceuticals
3. Importance of
Monoclonal antibodies in Industries
4. Appreciate the use
of microorganisms in fermentation technology
Unit I
a) Brief introduction to Biotechnology with reference to
Pharmaceutical Sciences.
b) Enzyme Biotechnology- Methods of enzyme immobilization
and applications.
c) Biosensors- Working and applications of biosensors in
Pharmaceutical Industries. d) Brief
introduction to Protein Engineering.
e) Use of microbes in industry. Production of Enzymes-
General consideration - Amylase, Catalase, Peroxidase, Lipase, Protease,
Penicillinase.
f) Basic principles of genetic engineering.
Unit II
a) Study of cloning vectors, restriction endonucleases and
DNA ligase.
b) Recombinant DNA technology. Application of genetic
engineering in medicine.
c) Application of r DNA technology and genetic engineering
in the production of: i) Interferon ii) Vaccines- hepatitis- B iii)
Hormones-Insulin.
d) Brief introduction to PCR
Unit III
Types of immunity- humoral immunity, cellular immunity
a) Structure of Immunoglobulins
b) Structure and Function of MHC
c) Hypersensitivity reactions, Immune stimulation and Immune
suppressions.
d) General method of the preparation of bacterial vaccines,
toxoids, viral vaccine, antitoxins, serum-immune blood derivatives and other
products relative to immunity.
e) Storage conditions and stability of official vaccines
f) Hybridoma
technology- Production, Purification and Applications
g) Blood products and Plasma Substituties.
Unit IV
a) Immuno blotting techniques- ELISA, Western blotting,
Southern blotting.
b) Genetic organization of Eukaryotes and Prokaryotes
c) Microbial genetics including transformation,
transduction, conjugation, plasmids and transposons.
d) Introduction to Microbial biotransformation and
applications.
e) Mutation: Types of mutation/mutants.
Unit V
a) Fermentation methods and general requirements, study of
media, equipments, sterilization methods, aeration process, stirring.
b) Large scale production fermenter design and its various
controls.
c) Study of the production of - penicillins, citric acid,
Vitamin B12, Glutamic acid, Griseofulvin,
d) Blood Products: Collection, Processing and Storage of
whole human blood, dried human plasma, plasma Substituties.
Recommended
Books (Latest edition):
1. B.R. Glick and J.J. Pasternak: Molecular Biotechnology:
Principles and Applications of RecombinantDNA: ASM Press Washington D.C.
2. RA Goldshy et. al., : Kuby Immunology.
3. J.W. Goding: Monoclonal Antibodies.
4. J.M. Walker and E.B. Gingold: Molecular Biology and
Biotechnology by Royal Society of Chemistry.
5. Zaborsky: Immobilized Enzymes, CRC Press, Degraland,
Ohio.
6. S.B.
Primrose: Molecular Biotechnology
(Second Edition) Blackwell Scientific Publication.
7. Stanbury F., P., Whitakar A., and Hall J., S., Principles
of fermentation technology, 2nd edition, Aditya books Ltd., New Delhi
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